Recognition of intestinal epithelial HIF-1 activation by Pseudomonas aeruginosa
نویسندگان
چکیده
Nachiket J. Patel, Olga Zaborina, Licheng Wu, Yingmin Wang, Donald J. Wolfgeher, Vesta Valuckaite, Mae J. Ciancio, Jonathan E. Kohler, Olga Shevchenko, Sean P. Colgan, Eugene B. Chang, Jerrold R. Turner,* and John C. Alverdy* Departments of Surgery, Pathology, Proteomics, and Medicine, the University of Chicago, Chicago, Illinois; and Department of Anesthesia, Critical Care Medicine, Brigham Women’s Hospital, Harvard Medical School, Cambridge, Massachusetts
منابع مشابه
Recognition of intestinal epithelial HIF-1alpha activation by Pseudomonas aeruginosa.
Human intestinal epithelial cell monolayers (Caco-2) subjected to hypoxia and reoxygenation release soluble factors into the apical medium that activate the virulence of the opportunistic pathogen Pseudomonas aeruginosa to express the potent barrier-dysregulating protein PA-I lectin/adhesin. In this study, we defined the role of hypoxia-inducible factor (HIF)-1alpha in this response. We tested ...
متن کاملHypoxia Modulates Infection of Epithelial Cells by Pseudomonas aeruginosa
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen commonly associated with lung and wound infections. Hypoxia is a frequent feature of the microenvironment of infected tissues which induces the expression of genes associated with innate immunity and inflammation in host cells primarily through the activation of the hypoxia-inducible factor (HIF) and Nuclear factor kappaB (NF-κ...
متن کاملComponents of intestinal epithelial hypoxia activate the virulence circuitry of Pseudomonas.
We have previously shown that a lethal virulence trait in Pseudomonas aeruginosa, the PA-I lectin, is expressed by bacteria within the intestinal lumen of surgically stressed mice. The aim of this study was to determine whether intestinal epithelial hypoxia, a common response to surgical stress, could activate PA-I expression. A fusion construct was generated to express green fluorescent protei...
متن کاملActivation of the lectin pathway of complement in experimental human keratitis with Pseudomonas aeruginosa
PURPOSE Pseudomonas aeruginosa (P. aeruginosa) microbial keratitis (MK) is a sight-threatening disease. Previous animal studies have identified an important contribution of the complement system to the clearance of P. aeruginosa infection of the cornea. Mannose-binding lectin (MBL), a pattern recognition receptor of the lectin pathway of complement, has been implicated in the host defense again...
متن کاملC. elegans SWAN-1 Binds to EGL-9 and Regulates HIF-1-Mediated Resistance to the Bacterial Pathogen Pseudomonas aeruginosa PAO1
Pseudomonas aeruginosa is a nearly ubiquitous human pathogen, and infections can be lethal to patients with impaired respiratory and immune systems. Prior studies have established that strong loss-of-function mutations in the egl-9 gene protect the nematode C. elegans from P. aeruginosa PAO1 fast killing. EGL-9 inhibits the HIF-1 transcription factor via two pathways. First, EGL-9 is the enzyme...
متن کامل